Agrobacterium-Mediated Transformation of pMDC140 Plasmid Containing the Wheatwin2 Gene into the Tadong Rice Genome

Blast disease resulting from Magnaporthe oryzae fungal infection reduces annual rice yield by up to 30% globally. The wheatwin2 (wwin2) is a pathogenesis-related (PR) gene that encodes for PR-4 protein with chitinase properties capable of degrading chitin, major constituent certain cell walls. However, the potential wwin2 contribute M. resistance in unclear. This study reports construction pMDC140 vector carrying and its Agrobacterium-mediated transformation into Tadong genome. In brief, was synthesized integrated using Gateway cloning technology transformed Our results show promising high rate, more than 90% calli expressing β-glucuronidase (GUS), reporter marker. expression further confirmed quantitative real-time polymerase chain reaction. conclusion, pMDC140-wwin2 constructed, which had rate could consistently induce GUS genes rice. Data this beneficial subsequent vitro oryzae-infected field experiments confirm defense mechanism towards blast